MICROSCOPY
- Students are expected to know the parts of the microscope and their
functions (GO)
- Students are expected to demonstrate care, focusing techniques and safety
concerns (GO)
- Prepare, stain and observe a wet mount of a specimen (GO)
1.
2. More to come soon [top]
3. The following are instructions
for preparing a wet mount of of onion epithelium [top]
- Add
one drop of water to a slide
-
Carefully use a razor blade and/ or forceps to
obtain a sample of onion epithelium
- Place
the onion on the slide into the water drop
- Apply
the cover slip using the 45o angle technique
A.
B.
C.
- Apply a
single drop of stain in the center, on one edge of the cover slip
- Using
paper-towel or tissue paper, draw the stain into the wet mount by placing it
on the opposite edge of the cover slip, as previously explained
A.
B.
- View
specimen under low power on the light microscope.